Apolipoprotein L 1 (APOL1) Mouse Monoclonal Antibody [Clone ID: OTI5E10]

CAT#: CF812043

Carrier-free (BSA/glycerol-free) APOL1 mouse monoclonal antibody,clone OTI5E10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOL1 (RC211114, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOL1. Positive lysates LY418517 (100ug) and LC418517 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from HepG2 cell line by using anti-APOL1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5E10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-339 of human APOL1 (NP_003652) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.97 kDa
• Gene Name: apolipoprotein L1
• Database Link:
  NP_003652
  Entrez Gene 8542 Human
  UniProt ID O14791
• Background: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
• Synonyms: APO-L; APOL; APOL-I; FSGS4

Reference Data

• Protein Families: Secreted Protein, Transmembrane