NDUFB3 Mouse Monoclonal Antibody [Clone ID: OTI7F4]

CAT#: CF810666

Carrier-free (BSA/glycerol-free) NDUFB3 mouse monoclonal antibody,clone OTI7F4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDUFB3 (Cat# RC201988, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB3 (1:500) (Cat# TA810666). Positive lysates LY419291 (100ug) and LC419291 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7F4
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-98 of human NDUFB3 (NP_002482) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.2 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B3
• Database Link:
  NP_002482
  Entrez Gene 4709 Human
  UniProt ID O43676
• Synonyms: B12; CI-B12

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease