BAG5 Mouse Monoclonal Antibody [Clone ID: OTI4E12]

CAT#: CF810632

Carrier-free (BSA/glycerol-free) BAG5 mouse monoclonal antibody,clone OTI4E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAG5 (RC208518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAG5 (1:2000). Positive lysates LY417691 (100ug) and LC417691 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-BAG5 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4E12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BAG5 (NP_004864) produced in 293T.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BAG cochaperone 5
• Database Link:
  NP_004864
  Entrez Gene 70369 MouseEntrez Gene 366734 RatEntrez Gene 9529 Human
  UniProt ID Q9UL15
• Background: The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: BAG-5

Reference Data

• Protein Families: Druggable Genome