PTPN22 Mouse Monoclonal Antibody [Clone ID: OTI6B1]

CAT#: CF810177

Carrier-free (BSA/glycerol-free) PTPN22 mouse monoclonal antibody,clone OTI6B1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPN22 (Cat# RC218969, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN22 antibody (Cat# TA810177). Positive lysates LY414274 (100ug) and LC414274 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-PTPN22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810177) (1:1000)

Show all 13 images

Specifications

Product Data

• Clone Name: OTI6B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 359-449 of human PTPN22 (NP_057051) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91.5 kDa
• Gene Name: protein tyrosine phosphatase non-receptor type 22
• Database Link:
  NP_057051
  Entrez Gene 26191 Human
  UniProt ID Q9Y2R2
• Background: This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
• Synonyms: LYP; LYP1; LYP2; PEP; PTPN8

Reference Data

• Protein Families: Druggable Genome, Phosphatase