LONRF3 Mouse Monoclonal Antibody [Clone ID: OTI9G7]

CAT#: CF809447

Carrier-free (BSA/glycerol-free) LONRF3 mouse monoclonal antibody,clone OTI9G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LONRF3 (RC214236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LONRF3 (1:2000). Positive lysates LY400406 (100ug) and LC400406 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9G7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 206-456 of human LONRF3 (NP_001027026) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.3 kDa
• Gene Name: LON peptidase N-terminal domain and ring finger 3
• Database Link:
  NP_001027026
  Entrez Gene 79836 Human
  UniProt ID Q496Y0
• Background: The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. Multiple alternatively spliced transcript variants have been suggested, but their full length natures are not clear. [provided by RefSeq, Jul 2008]
• Synonyms: RNF127

Reference Data

• Protein Families: Druggable Genome, Protease