GALNT10 Mouse Monoclonal Antibody [Clone ID: OTI4F11]

CAT#: CF809431

Carrier-free (BSA/glycerol-free) GALNT10 mouse monoclonal antibody,clone OTI4F11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALNT10 (RC223834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALNT10 (1:2000). Positive lysates LY405019 (100ug) and LC405019 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-GALNT10 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GALNT10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809431) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GALNT10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809431) (1:500)

Specifications

Product Data

• Clone Name: OTI4F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-318 of human GALNT10(NP_938080) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.8 kDa
• Gene Name: polypeptide N-acetylgalactosaminyltransferase 10
• Database Link:
  NP_938080
  Entrez Gene 170501 RatEntrez Gene 171212 MouseEntrez Gene 55568 Human
  UniProt ID Q86SR1
• Background: This gene encodes a member of the GalNAc polypeptide N-acetylgalactosaminyltransferases. These enzymes catalyze the first step in the synthesis of mucin-type oligosaccharides. These proteins transfer GalNAc from UDP-GalNAc to either serine or threonine residues of polypeptide acceptors. The protein encoded by this locus may have increased catalytic activity toward glycosylated peptides compared to activity toward non-glycosylated peptides. [provided by RefSeq, Apr 2010]
• Synonyms: GALNACT10; PPGALNACT10; PPGANTASE10

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis