Repulsive Guidance Molecule C (HFE2) Mouse Monoclonal Antibody [Clone ID: OTI8F4]

CAT#: CF809341

Carrier-free (BSA/glycerol-free) HFE2 mouse monoclonal antibody,clone OTI8F4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HFE2 (Cat# RC220228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HFE2 (Cat# TA809341)(1:2000).

Specifications

Product Data

• Clone Name: OTI8F4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 36-263 of human HFE2(NP_998818) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hemojuvelin BMP co-receptor
• Database Link:
  NP_998818
  Entrez Gene 148738 Human
  UniProt ID Q6ZVN8
• Synonyms: HFE2A; HJV; JH; RGMC

Reference Data

• Protein Families: Transmembrane