NOBOX Mouse Monoclonal Antibody [Clone ID: OTI4C5]

CAT#: CF808379

Carrier-free (BSA/glycerol-free) NOBOX mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NOBOX (Cat# RC216621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOBOX (Cat# TA808379)(1:2000). Positive lysates LY421630 (100ug) and LC421630 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NOBOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808379) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NOBOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808379) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-NOBOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808379) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NOBOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808379) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NOBOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808379) (1:150)

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Specifications

Product Data

• Clone Name: OTI4C5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-271 of human NOBOX(NP_001073882) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.1 kDa
• Gene Name: NOBOX oogenesis homeobox
• Database Link:
  NP_001073882
  Entrez Gene 135935 Human
  UniProt ID O60393
• Background: This homeobox gene encodes a transcription factor that is thought to play a role in oogenesis. In mice, it is essential for folliculogenesis and regulation of oocyte-specific genes. Defects in this gene result in premature ovarian failure type 5. [provided by RefSeq, May 2011]
• Synonyms: OG-2; OG2; OG2X; POF5; TCAG_12042