WDR61 Mouse Monoclonal Antibody [Clone ID: OTI4D2]

CAT#: CF807592

Carrier-free (BSA/glycerol-free) WDR61 mouse monoclonal antibody, clone OTI4D2 (formerly 4D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61 (RC203963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR61. Positive lysates LY410745 (100ug) and LC410745 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4D2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 92-305 of human WDR61(NP_079510) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.4 kDa
• Gene Name: WD repeat domain 61
• Database Link:
  NP_079510
  Entrez Gene 66317 MouseEntrez Gene 363064 RatEntrez Gene 80349 Human
  UniProt ID Q9GZS3
• Background: WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]). [supplied by OMIM, Mar 2008]
• Synonyms: REC14; SKI8

Reference Data

• Protein Pathways: RNA degradation