SHARP1 (BHLHE41) Mouse Monoclonal Antibody [Clone ID: OTI10B11]

CAT#: CF806146

Carrier-free (BSA/glycerol-free) BHLHE41 mouse monoclonal antibody, clone OTI10B11 (formerly 10B11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHLHE41 (RC206882, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHLHE41. Positive lysates LY410724 (100ug) and LC410724 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BHLHE41 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806146)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BHLHE41 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806146)

Specifications

Product Data

• Clone Name: OTI10B11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-297 of human BHLHE41(NP_110389) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.3 kDa
• Gene Name: basic helix-loop-helix family member e41
• Database Link:
  NP_110389
  Entrez Gene 79365 Human
  UniProt ID Q9C0J9
• Background: This gene encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors. The encoded protein functions as a transcriptional repressor and as a regulator of molecular clock. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, May 2010]
• Synonyms: BHLHB3; DEC2; hDEC2; SHARP1

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal