MTOR Mouse Monoclonal Antibody [Clone ID: OTI5B3]

CAT#: CF805914

Carrier-free (BSA/glycerol-free) MTOR mouse monoclonal antibody, clone OTI5B3 (formerly 5B3)

Product Images

Human recombinant protein fragment corresponding to amino acids 1766-2144 of human MTOR (NP_004949) produced in E.coli.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MTOR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805914) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MTOR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805914)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MTOR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805914) (1:500)

Specifications

Product Data

• Clone Name: OTI5B3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1766-2144 of human MTOR(NP_004949) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 288.7 kDa
• Gene Name: mechanistic target of rapamycin kinase
• Database Link:
  NP_004949
  Entrez Gene 56717 MouseEntrez Gene 56718 RatEntrez Gene 2475 Human
  UniProt ID P42345
• Synonyms: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, ErbB signaling pathway, Glioma, Insulin signaling pathway, mTOR signaling pathway, Pathways in cancer, Prostate cancer, Type II diabetes mellitus