MRTFA Mouse Monoclonal Antibody [Clone ID: OTI3G7]

CAT#: CF805226

Carrier-free (BSA/glycerol-free) MKL1 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MKL1 (Cat# RC221212, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKL1(Cat# TA805226).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805226)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MKL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805226)

Specifications

Product Data

• Clone Name: OTI3G7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 165-397 of human MKL1(NP_065882) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: myocardin related transcription factor A
• Database Link:
  NP_065882
  Entrez Gene 57591 Human
  UniProt ID Q969V6
• Synonyms: BSAC; MAL; MRTF-A

Reference Data

• Protein Families: Transcription Factors