RSPO3 Mouse Monoclonal Antibody [Clone ID: OTI3A9]

CAT#: CF804635

Carrier-free (BSA/glycerol-free) RSPO3 mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RSPO3 (Cat# RC205016, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO3(Cat# TA804635). Positive lysates LY403201 (100ug) and LC403201 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RSPO3 mouse monoclonal antibody. (TA804635; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (TA804635; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (TA804635; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (TA804635; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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Specifications

Product Data

• Clone Name: OTI3A9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-272 of human RSPO3 (NP_116173) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: R-spondin 3
• Database Link:
  NP_116173
  Entrez Gene 72780 MouseEntrez Gene 498997 RatEntrez Gene 84870 Human
  UniProt ID Q9BXY4
• Background: This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]
• Synonyms: CRISTIN1; PWTSR; THSD2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein