APPBP1 (NAE1) Mouse Monoclonal Antibody [Clone ID: OTI1E9]

CAT#: CF804321

Carrier-free (BSA/glycerol-free) NAE1 mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAE1 (RC201326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAE1. Positive lysates LY422668 (100ug) and LC422668 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1E9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-274 of human NAE1 (NP_001018170) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.4 kDa
• Gene Name: NEDD8 activating enzyme E1 subunit 1
• Database Link:
  NP_001018170
  Entrez Gene 84019 RatEntrez Gene 234664 MouseEntrez Gene 8883 Human
  UniProt ID Q13564
• Background: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: A-116A10.1; APPBP1; HPP1; ula-1

Reference Data

• Protein Pathways: Alzheimer's disease