L Kynurenine Hydrolase (KYNU) Mouse Monoclonal Antibody [Clone ID: OTI1F1]

CAT#: CF804120

Carrier-free (BSA/glycerol-free) KYNU mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KYNU (RC201559, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KYNU. Positive lysates LY422334 (100ug) and LC422334 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KYNU mouse monoclonal antibody. (TA804120) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KYNU mouse monoclonal antibody. (TA804120) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI1F1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-216 of human KYNU (NP_001028170) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: kynureninase
• Database Link:
  NP_001028170
  Entrez Gene 116682 RatEntrez Gene 8942 Human
  UniProt ID Q16719
• Background: Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
• Synonyms: KYNUU

Reference Data

• Protein Families: Protease
• Protein Pathways: Metabolic pathways, Tryptophan metabolism