MURF3 (TRIM54) Mouse Monoclonal Antibody [Clone ID: OTI7F8]

CAT#: CF803871

Carrier-free (BSA/glycerol-free) TRIM54 mouse monoclonal antibody, clone OTI7F8 (formerly 7F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM54 (Cat# RC218075, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM54(Cat# TA803871). Positive lysates LY403171 (100ug) and LC403171 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIM54 mouse monoclonal antibody. (TA803871) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI7F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 166-400 of human TRIM54 (NP_115935) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tripartite motif containing 54
• Database Link:
  NP_115935
  Entrez Gene 57159 Human
  UniProt ID Q9BYV2
• Background: The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Synonyms: MURF; MURF-3; muRF3; RNF30