APC Mouse Monoclonal Antibody [Clone ID: OTI2B8]

CAT#: CF803655

Carrier-free (BSA/glycerol-free) APC mouse monoclonal antibody, clone OTI2B8 (formerly 2B8)

Product Images

Human recombinant protein fragment corresponding to amino acids 955-1382 of human APC (NP_001120983) produced in E.coli.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APC mouse monoclonal antibody. (TA803655; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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Specifications

Product Data

• Clone Name: OTI2B8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 955-1382 of human APC (NP_001120983) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 311.5 kDa
• Gene Name: APC regulator of WNT signaling pathway
• Database Link:
  NP_001120983
  Entrez Gene 11789 MouseEntrez Gene 24205 RatEntrez Gene 324 Human
  UniProt ID P25054
• Background: This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]
• Synonyms: BTPS2; DP2; DP2.5; DP3; GS; PPP1R46

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Regulation of actin cytoskeleton, Wnt signaling pathway