ZNF165 Mouse Monoclonal Antibody [Clone ID: OTI4C1]

CAT#: CF800423

Carrier-free (BSA/glycerol-free) ZNF165 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF165 (Cat# RC205600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF165(Cat# TA800423). Positive lysates LY418674 (100ug) and LC418674 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 165-485 of human ZNF165(NP_003438) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.6 kDa
• Gene Name: zinc finger protein 165
• Database Link:
  NP_003438
  Entrez Gene 7718 Human
  UniProt ID P49910
• Background: This gene encodes a member of the Kruppel family of zinc finger proteins. Members of this DNA-binding protein family act as transcriptional regulators. This gene is located within a cluster of zinc finger family members. The encoded protein may play a role in spermatogenesis. [provided by RefSeq, Jul 2008]
• Synonyms: CT53; LD65; ZSCAN7

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors