Placental lactogen (CSH1) Mouse Monoclonal Antibody [Clone ID: OTI2G4]

CAT#: CF506708

Carrier-free (BSA/glycerol-free) CSH1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CSH1 (Cat# RC223244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSH1(Cat# TA506708). Positive lysates LY411615 (100ug) and LC411615 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CSH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506708)

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Specifications

Product Data

• Clone Name: OTI2G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CSH1(NP_072166) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.6 kDa
• Gene Name: chorionic somatomammotropin hormone 1
• Database Link:
  NP_072166
  Entrez Gene 1442 Human
  UniProt ID P0DML2
• Synonyms: CSA; CSH2; CSMT; FLJ75407; PL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction