CD22 Mouse Monoclonal Antibody [Clone ID: OTI4C3]

CAT#: CF506412

Carrier-free (BSA/glycerol-free) CD22 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22 (RC216939, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD22.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506412)

Specifications

Product Data

• Clone Name: OTI4C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD22 molecule
• Database Link:
  NP_001762
  Entrez Gene 12483 MouseEntrez Gene 308501 RatEntrez Gene 476485 DogEntrez Gene 106994739 MonkeyEntrez Gene 933 Human
  UniProt ID P20273
• Synonyms: SIGLEC-2; SIGLEC2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Cell adhesion molecules (CAMs), Hematopoietic cell lineage