SAPK4 (MAPK13) Mouse Monoclonal Antibody [Clone ID: OTI12C12]

CAT#: CF505805

Carrier-free (BSA/glycerol-free) MAPK13 mouse monoclonal antibody, clone OTI12C12 (formerly 12C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK13 (RC200606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK13. Positive lysates LY400973 (100ug) and LC400973 (20ug) can be purchased separately from OriGene.

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  Anti-MAPK13 mouse monoclonal antibody (TA505805) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK13 (RC200606).

Specifications

Product Data

• Clone Name: OTI12C12
• Applications: IF, WB
• Recommended Dilution: WB 1:4000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAPK13(NP_002745) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.9 kDa
• Gene Name: mitogen-activated protein kinase 13
• Database Link:
  NP_002745
  Entrez Gene 26415 MouseEntrez Gene 29513 RatEntrez Gene 5603 Human
  UniProt ID O15264
• Background: This gene encodes a member of the mitogen-activated protein (MAP) kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The encoded protein is a p38 MAP kinase and is activated by proinflammatory cytokines and cellular stress. Substrates of the encoded protein include the transcription factor ATF2 and the microtubule dynamics regulator stathmin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
• Synonyms: MAPK-13; MAPK 13; p38delta; PRKM13; SAPK4

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway