PCDH7 Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: CF505443

Carrier-free (BSA/glycerol-free) PCDH7 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH7 (RC223033, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH7. Positive lysates LY410093 (100ug) and LC410093 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-PCDH7 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505443)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505443)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505443)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505443)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505443)

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Specifications

Product Data

• Clone Name: OTI1F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PCDH7(NP_115833) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 132.4 kDa
• Gene Name: protocadherin 7
• Database Link:
  NP_115833
  Entrez Gene 54216 MouseEntrez Gene 5099 Human
  UniProt ID O60245
• Background: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120

Reference Data

• Protein Families: Druggable Genome, Transmembrane