TRIM22 Mouse Monoclonal Antibody [Clone ID: OTI1D11]

CAT#: CF505281

Carrier-free (BSA/glycerol-free) TRIM22 mouse monoclonal antibody, clone OTI1D11 (formerly 1D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM22 (Cat# RC207431, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM22(Cat# TA505281). Positive lysates LY401829 (100ug) and LC401829 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505281)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505281)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505281)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TRIM22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505281)

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  Anti-TRIM22 mouse monoclonal antibody (TA505281) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM22 (RC207431).

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Specifications

Product Data

• Clone Name: OTI1D11
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 61-406 of human TRIM22(NP_006065) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.8 kDa
• Gene Name: tripartite motif containing 22
• Database Link:
  NP_006065
  Entrez Gene 10346 Human
  UniProt ID Q8IYM9
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
• Synonyms: GPSTAF50; RNF94; STAF50

Reference Data

• Protein Families: Druggable Genome, Transcription Factors