C1S Mouse Monoclonal Antibody [Clone ID: OTI4E3]

CAT#: CF504082

Carrier-free (BSA/glycerol-free) C1S mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C1S (RC218963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C1S. Positive lysates LY400655 (100ug) and LC400655 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-C1S mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504082)

• Anti-C1S mouse monoclonal antibody (TA504082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C1S (RC218963).

• Flow cytometric Analysis of Jurkat cells, using anti-C1S antibody (TA504082), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Hela cells, using anti-C1S antibody (TA504082), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C1S(NP_001725) produced in HEk293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.8 kDa
• Gene Name: complement C1s
• Database Link:
  NP_001725
  Entrez Gene 716 Human
  UniProt ID P09871
• Background: This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq]
• Synonyms: FLJ44757

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus