CCM2 Mouse Monoclonal Antibody [Clone ID: OTI2F9]

CAT#: CF503414

Carrier-free (BSA/glycerol-free) CCM2 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCM2 (RC201418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCM2. Positive lysates LY410516 (100ug) and LC410516 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503414)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503414)

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  Anti-CCM2 mouse monoclonal antibody (TA503414) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CCM2 (RC201418).

Specifications

Product Data

• Clone Name: OTI2F9
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CCM2(NP_113631) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.7 kDa
• Gene Name: CCM2 scaffold protein
• Database Link:
  NP_113631
  Entrez Gene 216527 MouseEntrez Gene 305505 RatEntrez Gene 83605 Human
  UniProt ID Q9BSQ5
• Background: This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.
• Synonyms: C7orf22; OSM; PP10187