DOK2 Mouse Monoclonal Antibody [Clone ID: OTI3D6]

CAT#: CF503337

Carrier-free (BSA/glycerol-free) DOK2 mouse monoclonal antibody, clone OTI3D6 (formerly 3D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (RC207621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2. Positive lysates LY418321 (100ug) and LC418321 (20ug) can be purchased separately from OriGene.

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  Anti-DOK2 mouse monoclonal antibody (TA503337) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2 (RC207621).

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  HEK293T cells transfected with either RC207621 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody (TA503337), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3D6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DOK2(NP_003965) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.2 kDa
• Gene Name: docking protein 2
• Database Link:
  NP_003965
  Entrez Gene 9046 Human
  UniProt ID O60496
• Background: The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq]
• Synonyms: p56DOK; p56dok-2

Reference Data

• Protein Families: Druggable Genome