VCAM1 Mouse Monoclonal Antibody [Clone ID: OTI5B12]

CAT#: CF502392

Carrier-free (BSA/glycerol-free) VCAM1 mouse monoclonal antibody, clone OTI5B12 (formerly 5B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Positive lysates LY421491 (100ug) and LC421491 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502392)

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  Anti-VCAM1 mouse monoclonal antibody (TA502392) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1 (RC209761).

Specifications

Product Data

• Clone Name: OTI5B12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VCAM1(NP_001069) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.7 kDa
• Gene Name: vascular cell adhesion molecule 1
• Database Link:
  NP_001069
  Entrez Gene 7412 Human
  UniProt ID P19320
• Background: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: CD106; INCAM-100

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration