SULT2A1 Mouse Monoclonal Antibody [Clone ID: OTI7G10]

CAT#: CF501693

Carrier-free (BSA/glycerol-free) SULT2A1 mouse monoclonal antibody, clone OTI7G10 (formerly 7G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SULT2A1 (RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1. Positive lysates LY418856 (100ug) and LC418856 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-SULT2A1 monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI7G10
• Applications: WB
• Recommended Dilution: WB: 1:200 - 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SULT2A1 (NP_003158) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: sulfotransferase family 2A member 1
• Database Link:
  NP_003158
  Entrez Gene 6822 Human
  UniProt ID Q06520
• Background: This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq]
• Synonyms: DHEA-ST; DHEA-ST8; DHEAS; HST; hSTa; ST2; ST2A1; ST2A3; STD; SULT2A3