COX6A1 Mouse Monoclonal Antibody [Clone ID: OTI4G2]

CAT#: CF501431

Carrier-free (BSA/glycerol-free) COX6A1 mouse monoclonal antibody, clone OTI4G2 (formerly 4G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY COX6A1 (Cat# RC210485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COX6A1 (Cat# TA501431). Positive lysates LY401392 (100ug) and LC401392 (20ug) can be purchased separately from OriGene.

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  Anti-COX6A1 mouse monoclonal antibody (TA501431) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COX6A1 (RC210485).

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  HEK293T cells transfected with either RC210485 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COX6A1 antibody (TA501431), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4G2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COX6A1 (NP_004364) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 9.6 kDa
• Gene Name: cytochrome c oxidase subunit 6A1
• Database Link:
  NP_004364
  Entrez Gene 1337 Human
  UniProt ID P12074
• Background: Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented. [provided by RefSeq]
• Synonyms: CMTRID; COX6A; COX6AL

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease