BHMT Mouse Monoclonal Antibody [Clone ID: OTI10B3]

CAT#: CF500960

Carrier-free (BSA/glycerol-free) BHMT mouse monoclonal antibody, clone OTI10B3 (formerly 10B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT. Positive lysates LY400644 (100ug) and LC400644 (20ug) can be purchased separately from OriGene.

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  Anti-BHMT mouse monoclonal antibody (TA500960) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT (RC203148).

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  Flow cytometric Analysis of Jurkat cells, using anti-BHMT antibody (TA500960), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-BHMT antibody (TA500960), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI10B3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.8 kDa
• Gene Name: betaine--homocysteine S-methyltransferase
• Database Link:
  NP_001704
  Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 635 Human
  UniProt ID Q93088
• Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
• Synonyms: BHMT1; HEL-S-61p

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways