HSP90AA1 Mouse Monoclonal Antibody [Clone ID: OTI3B5]

CAT#: CF500883

Carrier-free (BSA/glycerol-free) HSP90AA1 mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSP90AA1 (Cat# RC212496, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSP90AA1(Cat# TA500883). Positive lysates LY417365 (100ug) and LC417365 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-HSP90AA1 monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI3B5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HSP90AA1 (NP_005339) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84.7 kDa
• Gene Name: heat shock protein 90 alpha family class A member 1
• Database Link:
  NP_005339
  Entrez Gene 15519 MouseEntrez Gene 299331 RatEntrez Gene 3320 Human
  UniProt ID P07900
• Background: HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding,protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria
• Synonyms: EL52; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Antigen processing and presentation, NOD-like receptor signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer