IRF3 Mouse Monoclonal Antibody [Clone ID: OTI5D2]

CAT#: CF500465

Carrier-free (BSA/glycerol-free) IRF3 mouse monoclonal antibody, clone OTI5D2 (formerly 5D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IRF3 (Cat# RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (Cat# TA500465).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-IRF3monoclonal antibody.

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  Western blot analysis of extracts (35ug) from 4 different cell lines and human plasma by using anti-IRF3 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and IRF3-Knockout HeLa cells (KO, Cat# LC833375) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody TA500465 (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500465, Dilution 1:50)

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Specifications

Product Data

• Clone Name: OTI5D2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000 IHC 1:50
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human IRF3 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.2 kDa
• Gene Name: interferon regulatory factor 3
• Database Link:
  NP_001562
  Entrez Gene 718970 MonkeyEntrez Gene 3661 Human
  UniProt ID Q14653
• Background: IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
• Synonyms: interferon regulatory factor 3; MGC94729

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway