Apolipoprotein M (APOM) Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF500074

Carrier-free (BSA/glycerol-free) APOM mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY APOM (Cat# RC204679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOM(Cat# TA500074). Positive lysates LY402735 (100ug) and LC402735 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-APOM monoclonal antibody at 1:200 dilution.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-APOM monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-APOM (TA500074) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI3H3
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC 1;50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 23-188 of human APOM
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.1 kDa
• Gene Name: apolipoprotein M
• Database Link:
  NP_061974
  Entrez Gene 55937 Human
  UniProt ID O95445
• Background: The APOM is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized
• Synonyms: apo-M; G3a; HSPC336; NG20

Reference Data

• Protein Families: Secreted Protein