Collagen VI (COL6A1) Rabbit Polyclonal Antibody

CAT#: BP8033S

Collagen VI (COL6A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA: 1/200 (OD ≥ 500).
  RIA.
  Immunofluorescence: 1/80.
  Immunohistology on Frozen and Paraffin Embedded Sections.
  For paraffin: 1/400-1/1000, 60 min at RT or 2-8°C over night for human collagen type VI, lower dilution is recommended for other species).
  Positive Control: Human placenta.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Bovine, Chicken, Human, Mouse, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type VI from human placenta.
• Specificity: Human Collagen type VI: 100% binding in RIA at 1/10,000 dilution.
  Cross-reactivity:
  Human Collagen type I, II, III, IV and V: < 0.1%.
  Human Elastin: < 0.1%.
  Human Laminin: < 0.1%.
  Human Fibronectin: < 1%.
• Formulation: PBS containing no BSA or preservative.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 0.1 distilled water. For further dilution use appropriate antibody diluent.
• Concentration: 1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type VI alpha 1
• Database Link:
  Entrez Gene 12833 MouseEntrez Gene 294337 RatEntrez Gene 1291 Human
  UniProt ID P12109
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.
• Synonyms: COL6A1

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion