COL3A1 Rabbit Polyclonal Antibody

CAT#: BP8013

COL3A1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA.
  RIA.
  Western blot.
  Immunofluorescence: 1/40.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Incubation Time: 60 min at RT or 2-8°C over night (please see also "Protocols").
  Positive Control: chicken skin or liver.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type III from Chicken skin.
• Specificity: Chicken Collagen type III: 100%
  Chicken Collagen type I: < 5 %.
  Cross-reaction with Human, Mouse, Rat, Bovine Collagen Type III: < 0,1 %.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.1 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen, type III, alpha 1
• Database Link:
  Entrez Gene 396340 Chicken
  UniProt ID P12105
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
• Synonyms: COL3A1