Collagen IV (COL4A1) Rabbit Polyclonal Antibody

CAT#: BP5031

Collagen IV (COL4A1) rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot (Immunoblotting).
  ELISA (Reacts exclusively with Collagen IV).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: Proteolytic treatment required (Enzyme: Pepsin).
  Recommended Positive Control: Skin, Kidney.
  Working Dilution: 1/50-1/100.
  Incubation Time: 1 h at RT or overnight at 2-8°C.
• Reactivities: Canine, Feline, Fish, Human, Mouse, Porcine, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human placental type IV Collagen.
• Specificity: The antibody is reactive with Collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antiserum is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology.
  In Immunohistochemistry no cross-reactivity with other Collagens at optimal dilutions.
  In Immunoblotting, a slight cross-reactivity with Collagen type V is observed.
• Formulation: Liquid stabilized antiserum with 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type IV alpha 1 chain
• Database Link:
  Entrez Gene 12826 MouseEntrez Gene 290905 RatEntrez Gene 1282 Human
  UniProt ID P02462
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
• Synonyms: COL4A1

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer