Apolipoprotein A I (APOA1) Mouse Monoclonal Antibody [Clone ID: G2]

CAT#: BM785

Apolipoprotein A I (APOA1) mouse monoclonal antibody, clone G2, Purified

Specifications

Product Data

• Clone Name: G2
• Applications: ELISA
• Recommended Dilution: ELISA (1/2.500-1/10.000). This antibody is suitable for coating microtitre plates in a Sandwich ELISA using catalogue number BP912HRP for detection.
  Western Blot (1/250-1/1000).
  Immunohistochemistry on Frozen Sections (1/20-1/80).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Native Human Apolipoprotein A1 from Human plasma.
• Specificity: Reacts with both free Apolipoprotein A1 and HDL bearing Apo-A1. Does not cross-react with ApoE, ApoB or Albumin.
• Formulation: 0.01M Sodium Phosphate, 0.01M Sodium Borate, 0.15M Sodium Chloride with 1% Mannitol and 1% Dextran.
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1.0 ml distilled water.
  Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the lyophilized antibody at 2-8°C and after reconstitution at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: apolipoprotein A1
• Database Link:
  Entrez Gene 335 Human
  UniProt ID P02647
• Background: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease.
  The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
• Synonyms: APOA1, ApoA-I, Apo-AI, ApoAI