Sele (CR1 Region) Rat Monoclonal Antibody [Clone ID: UZ7]

CAT#: BM6035P

Sele (CR1 Region) rat monoclonal antibody, clone UZ7, Purified

Specifications

Product Data

• Clone Name: UZ7
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunoblotting.
  Immunoprecipitation.
  Flow Cytometry (1/1000).
  Immunohistochemistry on Fozen Sections.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
• Specificity: UZ7 recognizes a conformational epitope in the CR1 region in murine E-selectin.
  UZ7 partially inhibits leukocyte adhesion to murine activated endothelial cells.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: selectin, endothelial cell
• Database Link:
  Entrez Gene 20339 Mouse
  UniProt ID Q00690
• Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
• Synonyms: SELE, ELAM1, LECAM2