GNRHR Mouse Monoclonal Antibody [Clone ID: A9E4]

CAT#: BM582S

GNRHR mouse monoclonal antibody, clone A9E4, Purified

Specifications

Product Data

• Clone Name: A9E4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the amino acids 1-29 of the Human GnRH receptor extracellular domain conjugated to BSA.
  Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.
• Specificity: This Monoclonal antibody A9E4 reacts with GnRH receptors in the anterior pituitary.
  A9E4 showed specificity for the synthetic peptide in ELISA and Dot blot, it has a higher affinity for the antigen than the GnRH receptor antibody clone F1G4 (Cat.-No. AM32064PU-N).
  A9E4 specifically stains human GnRH receptors on frozen tissue of the anterior pituitary.
  Recognition was supported by Western blotting.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: gonadotropin releasing hormone receptor
• Database Link:
  Entrez Gene 2798 Human
  UniProt ID P30968
• Background: Gonadotropin releasing hormone (GnRH) receptor is a protein of 54-60 kDa. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulated hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.
  
• Synonyms: Gonadotropin-releasing hormone receptor, GnRH-R, GRHR