gamma Catenin (JUP) Mouse Monoclonal Antibody [Clone ID: PG 5.1]

CAT#: BM5100

gamma Catenin (JUP) mouse monoclonal antibody, clone PG 5.1, Purified

Specifications

Product Data

• Clone Name: PG 5.1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Immunoblotting.
  Immunoprecipitation.
  Immunofluorescence Microscopy: 1/5 (with PBS, pH 7.4).
  Immunohistochemistry on Frozen Sections.
  Incubation Time: 1 h at RT.
  Immunohistochemistry on Paraffin Sections has been described (See reference product citation 1, Brodehl, et al, 2013 ).
• Reactivities: Bovine, Chicken, Human, Mouse, Rat, Zebrafish
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Plakoglobin, "band 5" protein from Bovine snout epidermis
• Specificity: PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells); Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin.
  Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin).
  Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).
• Formulation: Final solution contains PBS buffer, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in 1 ml distilled water
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: junction plakoglobin
• Database Link:
  Entrez Gene 3728 Human
  UniProt ID P14923
• Background: Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.
• Synonyms: Desmoplakin-3, Desmoplakin III, Catenin gamma, JUP, CTNNG, DP3