Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]
Vimentin (VIM) mouse monoclonal antibody, clone V9, Purified
|Applications||FC, IF, IHC, WB|
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin-Embedded Sections.
Recommended Dilutions: 1/25–1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
|Reactivity||Chicken, Human, Rat, Sheep, Potoroo|
|Immunogen||V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.|
|Specificity||The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.|
|Formulation||PBS with 0.09% Sodium Azide as preservative
State: Liquid purified IgG fraction
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
|Stability||Shelf life: one year from despatch.|
|Background||Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.|