Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: V9]

CAT#: BM5050P

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Vimentin (VIM) mouse monoclonal antibody, clone V9, Purified



USD 390.00


Availability*
2 Weeks

Size
    • 100 ug


Product images

Specifications

Product Data
Clone Name V9
Applications FC, IF, IHC, WB
Recommended Dilution Immunoblotting. 
Flow Cytometry.
Immunocytochemistry. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin-Embedded Sections. 
Recommended Dilutions1/25–1/200 for Flow Cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Reactivity Chicken, Human, Rat, Sheep, Potoroo
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.
Specificity The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Formulation PBS with 0.09% Sodium Azide as preservative
State: Purified
State: Liquid purified IgG fraction
Concentration lot specific
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Synonyms VIM
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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