Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: M20]

CAT#: BM5045P

Cytokeratin 8 (KRT8) mouse monoclonal antibody, clone M20, Purified

Product Images

Immunohistochemistry on Frozen Section of Human colon

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  Immunohistochemistry on Paraffin Section of Human colon

Specifications

Product Data

• Clone Name: M20
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Immunoblotting: 1/100-1/1000
  Flow Cytometry.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Tissues: 1/100-1/200 with Avidin-biotinylated Horseradish peroxidase complex ABC as detection reagent.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Human, Mammalian, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Keratin isolated from the Human breast carcinoma cell line MCF-7
• Specificity: M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.
  The M20 epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of Cytokeratin 8 (See Reference 10).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: keratin 8
• Database Link:
  Entrez Gene 3856 Human
  UniProt ID P05787
• Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are numbered 1 to 20.
  The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
  Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A