Lamin B1 (LMNB1) Mouse Monoclonal Antibody [Clone ID: 119D5-F1]

CAT#: BM4501

Lamin B1 (LMNB1) mouse monoclonal antibody, clone 119D5-F1, Purified

Product Images

Immunohistochemistry: Lamin B1 antibody Immunostaining of Human epidermis.

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  Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

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  Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.

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  Immunofluorescence staining of a 9 days old Zebrafish embryos. Frozen sectionfixed in Acteone/Methanol: 1/1. diluted at 1/100, incubated for 45 minutes at RT.

Specifications

Product Data

• Clone Name: 119D5-F1
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Flow cytometry: 1/100-1/200.
  Immunohistochemistry on Frozen Sections.
  Immunocytochemistry: 1/100-1/200 with Avidin-Biotinylated Horseradish peroxidase complex (ABC) as detection reagent.
  Western blot: 1/100-1/1000.
• Reactivities: Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Rat liver lamins.
  119D5-F1 is a Mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse.
• Specificity: The antibody Clone 119D5-F1 reacts with an epitope located C-terminal of residue 231 in Lamin B1.
• Formulation: PBS containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 67 kDa (Predicted)
• Gene Name: lamin B1
• Database Link:
  Entrez Gene 4001 Human
  UniProt ID P20700
• Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
  Lamins play a crucial role in the maintenance of nuclear structure, gene regulation, and signaling from cytoplasm to nucleus. Mutations in lamins have been associated with a number of pathologies; the so-called laminopathies.
• Synonyms: Lamin-B1, LMNB1, LMNB-1, LMN2, LMNB, Nuclear Envelope Marker