p53 (TP53) (16-25) Mouse Monoclonal Antibody [Clone ID: BP53-12]
p53 (TP53) (16-25) mouse monoclonal antibody, clone BP53-12, FITC
|Recommended Dilution||Immunofluorescence: This conjugate is routinely tested by Flow Cytometry analysis using permeabilized transient wild-type p53-tranfectants.|
|Immunogen||Bacterially expressed full-length wild-type p53.|
|Specificity||This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.|
|Formulation||Phosphate buffered saline (PBS), pH~7.4
State: Liquid purified Ig fraction
Preservative: 15 mM Sodium Azide
Label: Fluorescein Isothiocyanate.
The reagent is free of unconjugated FITC.
|Storage||Store the antibody undiluted at 2-8 °C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
|Stability||Shelf life: one year from despatch.|
|Background||The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.|
|Synonyms||Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13|
|The use of this Antibodies has been cited in the following citations:|
Epitope analysis of the human p53 tumour suppressor protein
,DolezalovC! H, VojtDsek B, KovarC-k J,
Folia Biol. (Praha) 1997
,PubMed ID 9158951
p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia
,BC!rtkovC! J, BC!rtek J, LukC!s J, VojtDsek B, StaskovC! Z, Rejthar A, KovarC-k J, Midgley CA, Lane DP,
Int. J. Cancer 1991
,PubMed ID 1652567
Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies
,BC!rtek J, BC!rtkovC! J, VojtDsek B, StaskovC! Z, LukC!s J, Rejthar A, KovarC-k J, Midgley CA, Gannon JV, Lane DP,
,PubMed ID 1923535