Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: CIV22]

CAT#: BM4067

Collagen IV (COL4A1) mouse monoclonal antibody, clone CIV22, Purified

Product Images

Swine Kidney, Paraffin Section stained with type IV Collagen Antibody (Clone CIV22)

• 

  Human Tonsil, Paraffin Section stained with type IV Collagen Antibody (Clone CIV22)

Specifications

Product Data

• Clone Name: CIV22
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 0.125 µg/ml (1/1600).
  Immunohistochemistry on Paraffin Sections: 0.5 µg/ml (1/400). Proteinase K pretreatment for antigen retrieval is recommended.
  Also suitable for ELISA.
  Recommended Positive Control: Human tonsil.
  The antigen is stable to formalin fixation, however.
• Reactivities: Bovine, Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified pepsin fragments of Human type IV Collagen from Human kidneys.
• Specificity: This Monoclonal antibody Clone CIV22 stains basement membranes in a variety of tissues and organs, including kidney, skin, striated and smooth muscle, spleen, lymph node, lung, placenta, and tendon. It recognizes native, but not denatured Collagen type IV but nevertheless reacts
  clearly with formalin-fixed paraffin-embedded sections.
  CIV22 is a useful marker for monitoring destruction of basal membranes as observed in malignant neoplastic diseases.
  This antibody recognizes a conformational epitope on a helical part of native Collagen IV and loses its reactivity on denaturing the Collagen IV protein.
  Antigen Distribution on Tissue Sections: Basically all basement membranes except corneal epithelium.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction.
  Production: This antibody was produced Serum-free, without Fetal Calf Serum.
  Stabilizer: 5 mg/ml BSA
  Preservative: 0.05% (v/v) Kathon CG
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: 0.2 mg/ml (after reconstitution)
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: collagen type IV alpha 1 chain
• Database Link:
  Entrez Gene 1282 Human
  UniProt ID P02462
• Background: Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
• Synonyms: COL4A1
• Note: Protocol: Protocol with frozen, ice-cold acetone-fixed sections:
  The whole procedure is performed at room temperature
  1. Wash in PBS
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum.
  
  Protocol with formalin-fixed, paraffin-embedded sections:
  The whole procedure is performed at room temperature
  1. Deparaffinize and rehydrate tissue section
  2. Block endogenous peroxidase
  3. Wash in PBS
  4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
  5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
  6. Wash in PBS
  7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber
  8. Wash in PBS
  9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
  10. Wash in PBS
  11. Counterstain with Mayer's hemalum.