Macrophages Mouse Monoclonal Antibody [Clone ID: PM-2K]

CAT#: BM4037B

Macrophages mouse monoclonal antibody, clone PM-2K, Biotin conjugated

Product Images

Immunohistochemistry on frozen sections: Clone RM2K (Biotin) on Human tonsil.

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  Immunohistochemistry on frozen sections: Clone RM2K (Biotin) on Human tonsil.

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  Comparison of Staining Patterns of X-4, X-14 and PM-2K on different cell types

Specifications

Product Data

• Clone Name: PM-2K
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on frozen section: 0.2 mg/ml (1:1000).
  Suggested positive control: Human tonsil.
  Does not react on routinely processed paraffin sections.
• Reactivities: Bovine, Canine, Feline, Human, Monkey, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cultured human peritoneal macrophages
• Specificity: This antibody detects Macrophages.
  Antigen distribution: Isolated cells: Positive on >90% of alveolar macrophages, and on 10% of adherent peritoneal cells after 1 day culture. Negative on dendritic cells, on freshly isolated blood monocytes or peritoneal cells; and on bone marrow cells including monocytes, myelomonocytic precursors and megakaryocytes.
  Tissue sections: PM-2K stains most tissue macrophages in lymphoreticular organs such as thymus, spleen, lymph node and tonsil. It is positive on Kupffer cells of the liver, alveolar macrophages an d macrophages in the interstitial tissues of the kidney, pancreas and many other organs. Proliferating macrophages are positive i n Gaucher's disease , multicentric reticulohistiocytosis and malignant histiocytosis. In MFH infiltrating macrophages are staine d but not tumour cells. Some bone stromal cells but not osteoclast - like multinucleated giant cells are stained in GCT. Microglial cells, osteoclasts and dendritic cells such as Langerhans cells, interdigitating cells and follicular dendritic cells are nega tive.
• Formulation: Phosphate buffered saline pH 7.2 (PBS)
  State: Purified
  State: Lyophilized affinity purified Ig fraction
  Stabilizer: 5 mg/ml bovine serum albumin (BSA)
  Preservative: 0.05 % (v/v) Kathon CG
• Reconstitution Method: Reconstitute by adding 0.5 ml distilled water.
• Purification:
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Background: Monoclonal antibody PM-2K, together with X-4 and X-14, forms a particular group of macrophage specific antibodies which were tested at the Vth Leukocyte Typing Workshop. The PM-2K antigen was initially suspected to function as a scavenger receptor. However, experiments with CHO cells expressing Macrophage Scavenger Receptor (MSR) AI or AII showed no reactivity with this antibody.
• Synonyms: Macrophage marker