Collagen II (COL2A1) Mouse Monoclonal Antibody [Clone ID: 2G2/49 (COLL-II)]

CAT#: BM332

Collagen II (COL2A1) mouse monoclonal antibody, clone 2G2/49 (COLL-II), Ascites

Specifications

Product Data

• Clone Name: 2G2/49 (COLL-II)
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/100-1/500.
  Western blotting: 1/1000-1/2000.
  Immunofluorescence: Neat-1/5. Suitable for use on Fresh, Frozen or Acetone fixed material. Mild pepsin digestion is recommended to enhance staining.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat, Sheep
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human cartilage specific CNBr-cleaved Collagen II
• Specificity: Reacts with both pepsin solubilised and CNBr-cleaved Human and Bovine Collagen type II.
  No-cross reactivity is seen with types I, III, V or IX.
  Species Cross-Reactivity: Rat, Bovine, Sheep, Mouse, Dog, Pig.
• Formulation: State: Ascites
  State: Liquid Ascites without preservatives
  Stabilizer: None
  Preservative: None
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type II alpha 1 chain
• Database Link:
  Entrez Gene 12824 MouseEntrez Gene 25412 RatEntrez Gene 1280 Human
  UniProt ID P02458
• Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues.
  Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
• Synonyms: COL2A1, Alpha-1 type II collagen

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion