Collagen I (COL1A1) Mouse Monoclonal Antibody [Clone ID: NFI/20]

CAT#: BM331

Collagen I (COL1A1) mouse monoclonal antibody, clone NFI/20, Purified

Specifications

Product Data

• Clone Name: NFI/20
• Applications: ELISA, IHC
• Recommended Dilution: ELISA: 1/500-1/2000.
  Immunohistochemistry on Frozen Sections: 1/10-1/40.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human Collagen type I from placenta.
• Specificity: This antibody clone NFI/20 is specific for Human Collagen I and does not recognize Collagen types II, III and IV.
• Formulation: Buffer: 0.1M Sodium Chloride, 0.01M Sodium Phosphate, 0.01M Sodium Borate, with 1% Mannitol and 1% Dextran.
  State: Purified
  State: Lyophilized Ig fraction.
• Reconstitution Method: Restore with 1 ml of sterile distilled water.
• Purification: Ammonium Sulphate Precipitation and DEAE-Cellulose Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type I alpha 1
• Database Link:
  Entrez Gene 1277 Human
  UniProt ID P02452
• Background: Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
  Mutations in the encoding gene are associated with osteogenesis imperfecta (Peng et al. 2012), Ehlers Danlos syndrome (Nuytinck et al. 2000) and idiopathic juvenile osteoporosis (Pocock et al. 1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihme et al. 1984).
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion