ICAM1 Mouse Monoclonal Antibody [Clone ID: MEM-111]

CAT#: BM2448PE

ICAM1 mouse monoclonal antibody, clone MEM-111, PE

Specifications

Product Data

• Clone Name: MEM-111
• Applications: FC
• Recommended Dilution: Flow cytometry: 20 µl reagent / 100 µl of whole blood or 10e6 cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Bovine, Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Raji human Burkitt's lymphoma cell line
• Specificity: The antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
  Label: PE
  State: Liquid Ig fraction
  Label: Conjugated with R-Phycoerythrin under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use
• Conjugation: PE
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: One year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
  UniProt ID P05362
• Background: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Synonyms: ICAM-1