IGF1 Receptor (IGF1R) (alpha chain) Mouse Monoclonal Antibody [Clone ID: 1H7]

CAT#: BM2226LE

IGF1 Receptor (IGF1R) (alpha chain) mouse monoclonal antibody, clone 1H7, Low Endotoxin

Product Images

Staining of human peripheral blood granulocytes with Mouse anti Human CD221-Low Endotoxin Cat,.-No (Clone 1H7).

Specifications

Product Data

• Clone Name: 1H7
• Applications: FC, FN, IHC, WB
• Recommended Dilution: Functional Assays.
  Western blotting.
  Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Paraffin Sections: 1/10-1/40. This product requires protein digestion pre-treatment of paraffin sections e.g trypsin or pronase. Use pancreas or placenta as histology positive control tissue.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human placental IGF-I receptor.
• Specificity: Clone 1H7 recognizes an epitope in the alpha subunits of CD221, demonstrated by Western blotting (Ref.1).
  Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (Ref.1)
• Formulation: PBS without preservatives or stabilizers
  State: Low Endotoxin
  State: Liquid (sterile filtered) purified IgG fraction from tissue culture supernatant
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: insulin like growth factor 1 receptor
• Database Link:
  Entrez Gene 3480 Human
  UniProt ID P08069
• Background: CD221, a 155 kD tyrosine kinase, is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin.
  The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins.
• Synonyms: IGF-I receptor, IGF1 Receptor